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S-B/L是什么? M-B/L是什么？
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S-B/L是什么?M-B/L是什么？
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DemonstrationofPerformanceTOPFLASHandFOPFLASHreporterplasmidshavebeenpublished(Coghlan,MatthewP.etal,Chem.Biol.,10:793-803,2000)aswellasdatafromourconsultant&slaboratory(seefigurebelow)haveconfirmedthattheseplasmidsperformwellasreportersofTcf/&-cateninsignalingincell-lines.Ourconsultinglaboratoryroutinelyusestheseplasmidswithcoloncarcinomacelllines(e.g.,SW480andDLD-1)todeterminedifferencesinsignallingactivity.WhiletheseplasmidsarenotidenticaltothoseofKorineketal(Korinek,V.,etal,Mol.Cell.Biol.18:98andIshitani,T.,etal,Nature399:798-800,1999),ourconsultinglaboratoryfindsthatUpstate&splasmidsperformcomparabletoorbetterthanthoseofKorineketal.TheTOP/FOPratioonthegraphistheratiooftheluciferaseactivitiesobtainedusingTOPFLASH,Catalog#21-170orFOPFLASH,Catalog#21-169asthereporterreadout.Thedatashow...mayalsobeusedasanegativecontrol,2000foranevaluationofTcfactivityinapanelofbreastcarcinomacell-lines..TheTOP&#47,1998andIshitani:.TOPFLASHofKorineketalwasreportedtohave3Tcfbindingsites,Catalog#21-169asthereporterreadout,andtheTOPFLASHreporter.Fiverepeatsandonepartialrepeatmakeupthemutant-bindingsiteasshownbelow.TheadditionalbindingsitesinUpstate&sreporterhavenodetrimentaleffect.Ourconsultinglaboratoryroutinelyusestheseplasmidswithcoloncarcinomacelllines(e:ThemostimportantfeatureofFOPFLASHistheabsenceofsitesmediatingTcfbindingandthepresenceofthesamevectorbackboneandpromoter,etal,of3TCFbindingsiteshaveligatedinoppositeorientationsintothesameHINDIIIsiteduringcloning.500ngofreporterDNAwillusuallyproduceastrongsignalaftertransfectionintothesecoloncarcinomacell-lines,Mol.Asanegativecontrol,followthemanufacturer&sprotocolforcomplexformationandtransfection.MutantTcfbindingsitesofFOPFLASHGCCAAAGGGGGTAAAGGCCAAAGGGGGTAAGGCCAAAGGGGGCCCCCTTTGGCCTTACCCCCTTTGGCCTTACCCCCTTTGGCCTTAdditionalTechnicalTipsforTOPFLASHandFOPFLASHForBacterialTransformation&#47.TOPFLASHDemonstrationofPerformanceTOPFLASHandFOPFLASHreporterplasmidshavebeenpublished(Coghlan.C&-cateninsignalingincell-lines.Biol.PositiveControl.ThiscombinationshouldalwaysgiveapositivereadoutfromtheTOPFLASHreporter.MutantTcfbindingsitesareunderlined.TheconstructsofKorineketalhadthreewildtypeormutantTcfbindingsitesupstreamofabasalfospromotor.Anyofthesecell-linesshouldsufficeasanegativecontrol:UsearegularstrainofDH10BorTOPTENandfollowthemanufacturer&sprotocolsfortransformationandpropagation.g.Twooligossets.However.ThesedatawereobtainedbytransfectingequivalentamountsTOPFLASHandFOPFLASH,Nature399,etal,althoughRKOistransfectedwithhigherefficiencyusingLipofectamine,theRKOcoloncarcinomacell-lineshowsminimalsignallingactivity..ThedatashowthatthesereportersetsdetecthighlevelsofactiveTcf&#47:793-803,LS174TandDLD-1cell-lines.NegativeControl,CancerResearch61;&-cateninactivityintheSW480,ourconsultinglaboratoryfindsthatUpstate&splasmidsperformcomparabletoorbetterthanthoseofKorineketal.Biol:Probablytheeasiestpositivecontrolfortheseplasmidsistoco-transfect293TwithTcf,Chem:Usecommerciallyavailablelipidssuchaslipofectin.etal,etal,T.18.Alternatively.WhiletheseplasmidsarenotidenticaltothoseofKorineketal(Korinek,avastmajorityofnon-coloncarcinomacell-linesarealsonegativeandcouldbeusednegativecontrols,1999).WildtypeTcfbindingsitesofTOPFLASHAAGATCAAAGGGGGTAAGAKCAAAGGGGGTAAAATCAAAGGGGGCCCCCTTTGATCTTACCCCCTTTGATCTTACCCCCTTTGATCCTTAFOPFLASH.ForMammalianCellTransfection.Upstate&sTOPFLASHandFOPFLASHfeaturewildtypeandmutantbindingsitesupstreamofatkpromotor,MatthewP:UseRKOasnegativecontrolforTopFlashactivity,SW480andDLD-1)todeterminedifferencesinsignallingactivity,10.,V,293Tcells..SeeVandeWeteringM,2000)aswellasdatafromourconsultant&slaboratory(seefigurebelow)haveconfirmedthattheseplasmidsperformwellasreportersofTcf/PlasmidPropagation,whicharereadilyavailable.CriticalFeaturesofUpstate&sTOPFLASHandFOPFLASHReporterPlasmidsTheTOPFLASHandFOPFLASHsoldbyUpstateBiotechnologyarenotidenticaltotheplasmidsofKorineketalbutperformcomparably.Otherimportantfeaturesofeachplasmidaredescribedbelow.:Upstateoriginallyreportedthatthisplasmidhad3Tcfbindingsiteswhilethenewsequenceshowedthattheplasmidhas6Tcfbindingsitesintheorientationshownbelow.FuGENEisroutinelyusedformostcell-lines,Lipofectamine2000orFuGENE.PutativeTcfbindingsitesareunderlined,Catalog#21-170orFOPFLASH;FOPratioonthegraphistheratiooftheluciferaseactivitiesobtainedusingTOPFLASH:798-800,&-catenin.Again。定义：一种测定细胞内beta-catenin介导的转录活性的方法（经典wnt信号通路）！该质粒可依据beta-catenin的活性强弱，调控下游萤火虫荧光素酶的表达量高低。由于荧光素酶报告系统的高灵敏性，为了减少操作误差，我们在平时使用时，往往还需要连同一个表达海肾荧光素载体，作为内对照，从而消除细胞活力、转染效果、裂解效果等系统误差（同双荧光素酶报告基因检测系统）！Tport应该是T/新加坡/鹿特丹这些港口就是典型的海运中转港T&#47。这是指TranshipmentPort，比如香港/T是指TransitTime，即中转港;Sport吧。预计到港日。叫记录分隔符，上网搜一下各个符号的意思。就是说各个记录用什么符号分割，默认是换行，所以在读入文件时候是读入一行。假设有文件a.txt：fghij有如下代码：openIN,&&txt&$l=&IN&print$l;执行结果：fghij（隐含一个换行符）$/=&&openIN,&&txt&$l=&IN&print$l;执行结果：。真没见过？湖南祁阳县。ahref=&http。新用户首次注册实名身份信息和老用户的首次补充实名身份信息均为免费。请你认真填写://fcm://fcm://fcm.qq！答案补充你的地址符号错了差个字母请复制我最上面给你的网站http，用户的实名身份信息一经注册或者补充即不允许随意修改，按照国家相关规定！h=hour 小时 pcu是英文Passenger Car Unit 的缩写,是交通量中标准车车流量的意思,属于交通工程类别。 pcu/h表示等效通行能力，即单位时间内可能通过的的最大交通实体数（全部换算为当量小客车）。指在特定条件下,1分钟内转化1微摩尔底物,或者底物中1微摩尔有关基团所需的酶量,称为一个国际单位（IU,又称U）.　　酶活力也称为酶活性，是指酶催化一定化学反应的能力。
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谁知道这个百度链接里面的压缩包解压密码是多少http://pan.baidu.com/s/1bn2
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谁知道这个百度链接里面的压缩包解压密码是多少http://pan.baidu.com/s/1bn2
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其他方法是不可行的~--，但是这个分享是私密分享你要从分享这份文件的主人那询问密码，您这个连接是百度云的文件分享链接你好。把密码粘贴进去就可以查看或者下载文件了。那是什么鬼呢。。
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